Confocal Raman Spectrometer (WITec Alpha 300R)
Configuration Information:
l 532 nm laser source
l Micro-confocal Raman spectrometer
l High-precision automatic sample stage
l Automatic spectrum database for comparison and recognition
Performance and Parameters:
l High precision and spatial resolution
l Fast confocal Raman imaging system with white light Kohler illumination and Zeiss objective lenses (×10, ×50, ×100) that can automatically focus and track surface scanning based on sample undulations
l Laser with a wavelength of 532 nm, maximum power of 75 mW, and precise power control down to 0.1 mW, with a minimum laser spot diameter of 300 nm
l Ultra-high luminous flux spectrometer (UHTS 300) optimized for visible light (420 nm-830 nm) with wave number accuracy better than 1 cm-1
l Fully automatic high-precision sample stage with a spatial resolution of 100 nm and capability for 3D mapping
l Advanced data processing software for peak splitting, fitting, unmixing, deduction, multi-component analysis, 2D/3D imaging, and statistical analysis of spectral parameters
Applications:
The Raman spectroscopic characteristics of samples are primarily applied for micro-area, in-situ, real-time, and non-destructive analysis in various fields:
l Earth Science: Identifying micro rock minerals, analyzing inclusion compositions, and studying fossil ultrastructure and composition.
l Life Sciences: Profiling subcellular structures and components, analyzing biological tissue sections, studying microbial cell clusters or macro biological tissues, isotope labeling of cell metabolism, and targeted component analysis.
l Medicine and Chemistry: Rapidly detecting specific ingredients in food and drugs, and monitoring chemical reaction processes in micro-areas.
l Materials Science: Analyzing the molecular structure and composition of crystalline, amorphous, liquid, and gaseous materials.
l Environmental Science: Precisely quantifying substances (e.g., microplastic particles, pollutants) in samples from oceans, lakes, and rivers, including water bodies, sediments, and biological tissues.
GC-ToF-MS (Agilent 8890-LECO Pegasus BT)
Configuration Information:
l Fine column split/non-split injection port
l 162-position automatic sampler
l StayCleanTM electron bombardment ion source (EI)
l Time-of-flight mass analyzer
l NIST spectral library
Performance and Parameters:
l High-frequency rapid detection, high fidelity full spectrum scanning, high resolution, and high sensitivity
l Low detection limit, automatic peak recognition, and automatic deconvolution
l Suitable for identifying molecular structures of trace/unknown components and performing quantitative calculations
l High throughput with an acquisition rate of 35,000 Hz, capable of collecting 18-20 data points for chromatographic peaks
l Full collection with no ion concentration discrimination, no mass funnel effect, and no missed sample information
l Sensitivity of 10fg octafluoronaphthalene OFN with an SN signal-to-noise ratio greater than 10
l Detection limit better than Pick's level (×10-12), IDL less than 5fg
l Quality range of 5 amu to 1000 amu, quantitative linear dynamic range of 0.01 to 50ng
l Data stability and traceability with the BT system recording all chemical information at an ultra-fast collection rate
l Third-generation non-target object dual-dimensional deconvolution technology for analyzing complex compound co-current interference information
l Spectral Filter screening function for quickly and accurately screening target compounds in large datasets
l TAF (Target Analyst Peak Finding) quantitative target screening function for user-friendly and intelligent target quantification
l Professional mass spectrometry database for automatic analysis and identification of components and molecular structures
Applications:
The GC-ToF-MS (Agilent 8890-LECO Pegasus BT) is a versatile and powerful instrument for analyzing and detecting organic molecules, particularly suitable for identifying and analyzing unknown and trace organic components in complex matrices.
l Earth science: Detecting and analyzing organic molecules in geological and environmental samples
l Life Sciences: Analyzing known and unknown components in cell or biological tissue samples, identifying trace metabolites, and performing quantitative metabolomics calculations
l Environmental science: Detecting and quantitatively analyzing organic and volatile substances in soil, water, groundwater, atmosphere, and indoor environments
l Food, Medicine, Petrochemical, Criminal Investigation: Identifying, quantifying, and controlling quality standards of various samples, including additives, hormones, active ingredients, drugs, and explosives
Fourier transform infrared microscope system
(Bruker Vertex 80V-Hyperion II-FPA)
Configuration Information:
l Vertex 80V vacuum infrared spectrometer
l Hyperion II infrared microscope
l FPA focal plane array detector
Performance and Parameters:
This instrument boasts high sensitivity, spectral resolution, and spatial resolution. It can quickly acquire single-point, one-dimensional, and two-dimensional infrared spectra of the sample's surface or interior. It also performs advanced data processing and spectral parameter mapping.
l Bruker VERTEX 80V with vacuum-active collimation UltraScan interferometer, vacuum optical path, spectral range of 8000-350 cm-1, resolution of 0.2 cm-1, scanning rate of 65 spectra/second, liquid nitrogen-cooled MCT detector (spectral range: 12000-600 cm-1).
l Infrared Microscope: Capable of transmission and reflection modes, with blade aperture for single-point spectral acquisition or point-by-point surface scanning. Liquid nitrogen-cooled MCT detector (range: 10000-600 cm-1).
l FPA Detector: 64 × 64 pixel matrix, single surface scan range of 170 × 170 μm², high-precision sample stage with 0.1 μm spatial accuracy and 75 mm × 50 mm movement range.
l Software: OPUS for spectral processing, including peak removal, fitting, unmixing, deduction, multi-component analysis, two-dimensional imaging of spectral parameters, and statistical analysis.
Applications:
This system is primarily used for micro-area, in-situ, real-time, and non-destructive infrared spectroscopic analysis in various fields:
l Earth Science: Rock mineral analysis, inclusion composition analysis, fossil ultrastructure and composition analysis.
l Life Sciences: Spectral and compositional analysis of biological tissues and subcellular structures, microbial cell clusters, and tissue pathology.
l Medicine and Chemistry: Microscopic particle analysis of food and drugs, rapid detection of specific components, and real-time monitoring of chemical reactions.
l Materials Science: Molecular structure and composition analysis of crystalline, amorphous, liquid, and gaseous materials.
l Environmental Science: Precise quantitative micro-area analysis of substances (e.g., microplastic particles, pollutants) in water, sediment, and biological tissue samples.
Simulator: lcy moon surface environments（self-developed）
Configuration Information:
l vacuum chamber
l Medium energy gas ion source
l VUV light source
l In situ Raman detection module
l In situ infrared detection module
Performance and Parameters:
l High vacuum degree: 10-5 Pa~105 Pa
l Temperature of the sample stage: -263 ℃~ +100 ℃
l VUV Continuum Range: 110 nm~400 nm
l Medium-energy gas ion source: The acceleration voltage of the ion source is adjustable within a range of 8-50 keV, featuring a beam spot diameter of 10 mm. Within this 10 mm beam spot diameter, the ion current exceeds 0.5 mA
l In situ Raman detection module: Features a 532 nm laser source, a microscopic objective with a magnification of 50 ×.
l In situ infrared detection module: Features an incident beam aperture of Φ30 mm, a microscope objective with a magnification of 15 ×.
Applications:
This device is used to simulate the extreme environment of extraterrestrial oceans on ice satellites represented by Europa, Enceladus, and Saturn's moon Titan. It has low temperature, radiation, and vacuum conditions, and can conduct a series of physical, chemical, biological, and kinetic experiments. Meanwhile, the device can achieve in-situ Raman and infrared spectroscopy detection.
35 MPa hydrothermal chemostat（self-developed）
Performance and Parameters:
|
Main parameters |
total volume |
9x 100 mL |
material quality |
C276 | |
|
structural style |
flange |
Heating method |
Oil bath heating | ||
|
working pressure |
30 MPa |
design pressure |
35 MPa | ||
|
end-use temperature |
50 ℃ |
design temperature |
100 ℃ | ||
|
sample |
Double valve high pressure sampling | ||||
|
The feeding tank |
V-001 |
Volume 60 L room temperature and normal pressure material 316 L | |||
|
mixing drum |
V-002 |
Volume 60L can be clamped heating normal pressure material 316L | |||
|
gas mass flow meter |
MF-01 |
0-200 ml/min tunable, medium oxygen. Including instantaneous flow rate and cumulative flow output. | |||
|
pump |
BP01-03 |
parameter declaration | |||
|
gathering tank |
Y01-09 |
1L atmospheric pressure material: glass bottle body with scale | |||
|
control |
Standard control box / split type / touch screen / computer, etc., and the control logic need to be explained | ||||
|
Equipment size |
L*D*W= 1650x1220x1875 mm | ||||
Applications:
This device is used to simulate the living environment of microorganisms in deep-sea conditions. The entire device comprises an intake system, a feeding system, a reaction vessel system, and a control system. It is utilized for cultivating microorganisms under high temperature and pressure conditions. The experiment allows for continuous culture, and multiple reaction incubators can be employed for comparative experiments. Jacket temperature control can accommodate both high and low temperature reactions. It enables the flow of the culture medium throughout the experiment under a high-pressure environment to achieve the cultivation of microorganisms.
Low-Temperature High-Pressure Hydrostatic Incubation System
Performance and Parameters:
l Maximum working pressure: 120 MPa, acceptance pressure ≮ 130 MPa
l Effective size of the module: Φ85x300 mm
l Chamber material: titanium alloy (TC4)
l Use medium: fresh water or seawater
l Pressurization system: pneumatic booster pump; air source pressure: ≮ 0.6 MPa
l Computer display: pressure curve, data acquisition, record and storage, display accuracy: 0.01 MPa
l Control accuracy Pressure control accuracy: ±1 %F.S; acquisition accuracy: ± 0.5 %
l Use environment: -10 ℃ ~40 ℃ (ice-free)
Applications:
The Low-Temperature High-Pressure Hydrostatic Incubation System can be used to simulate the pressure of deep-sea environments ranging from 0 to 11,000 meters, as well as replicate the static pressure environment of experimental samples at full sea depth. It employs a computer-controlled pneumatic pressurization system to supply liquid to three sets of static pressure cultivation kettles, enabling them to fulfill the function of mimicking deep-sea pressure environments. The device is equipped with features such as automatic pressure control, experimental data acquisition, display, and recording storage.
3D Holo-Tomographic Live Cell lmaging Microscope
Configuration information:
l Microscope section
l Fluorescent module section
l Cell Heating Incubation System
Performance and Parameters:
Microscope section
l Resolution: XY=200nm ，Z=400nm
l Field of view: 80um
l Field of view depth: 30um
l Tomography imaging rate: 0.5 fps 3D RI frame, fully automatic adjustment
l Microscope objective: 60X, dry mirror NA 0.8、WD 0.3mm
l Low energy excitation laser: 520nm
l Sample energy: 0.2mW/mm2
l Holographic live cell chromatography technology
l 360 degree rotating arm for tomographic scanning
l 115S holographic capture speed
l Steve holographic cell analysis software
Fluorescent module section
l Light source: High speed switching<100 μ s, lifespan>20000h per channel
l Resolution: XY=400nm
l Field of view: 90 × 90 μ m
l Fluorescence channel: FITC(green),TRITC(orange),and DAPI(blue) or Cy5(red)
l Imaging: 2D, 4D time lapse: (RI+fluo）
l Imaging rate: 3fps per channel
l Fluorescent Sample Selection: All fluorescent samples suitable for laser excitation can undergo high-resolution imaging; Select specific fluorescent dyes
Cell Heating Incubation System
l Temperature control range: room temperature+3 ℃ -45 ℃
l Temperature control accuracy: ± 0.1 ℃
l Applicability: Applicable to all upright/inverted microscopes on the market;
l Optional black panel for fluorescence imaging to avoid fluorescence quenching
l Built in LED light controlled by foot switch for easy observation of samples
l can add additional CO2/O2 and humidity control chambers
Applications:
The 3D Holo-Tomographic Live Cell lmaging Microscope is currently the world's first microscope system that integrates holographic imaging, 360-degree rotating light source tomography, and fluorescence imaging technologies. It employs a rotating light source to pass through culture dishes or glass slides, transmitting images through an optical path to a computer for real-time dynamic observation via software. This microscope can simultaneously achieve label-free 3D cell imaging and fluorescence-labeled imaging, seamlessly integrating these images and upgrading fluorescence imaging to a 3D level.
This instrument examines the effects of screened samples on cell morphology, growth, differentiation, apoptosis, metabolic pathways, and signal transduction, while maintaining cell structure and function intact. It gathers extensive relevant information in a single experiment, determining biological activity and potential toxicity. It is widely used in various fields such as cytology, microbiology, immunology, neurobiology, physiology, genetics, virology, biophysics, and pharmacology.
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